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18:56 Dec 1, 2017 |
English to Dutch translations [PRO] Medical - Medical: Instruments | |||||||
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| Selected response from: Barend van Zadelhoff Netherlands Local time: 10:23 | ||||||
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4 | Polymer Refine Red Detection™-systeem |
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polymer refine red detection system Polymer Refine Red Detection™-systeem Explanation: Het lijkt me dat dit je dit niet kunt vertalen zonder dat het onherkenbaar wordt. Het is ook geen heldere taalkundige boodschap, het is meer een fantasienaam. Daarnaast zie ik dat het in andere talen onvertaald wordt gelaten. Bovendien zie ik dat het een merknaam schijnt te zijn: Code Red – an Exciting Advance in High-Contrast Clinical IHC/ISH Leica Microsystems has announced the worldwide release of the new Bond Polymer Refine Red Detection™ system. This new system uses a Fast Red chromogen for high-contrast clinical staining and is optimized for use on the fully automated Bond™ advanced stainer. https://www.leica-microsystems.com/company/news-events/news-... Human lung. Immunohistochemical staining of malignant pulmonary carcinoma cells for thyroid transcription factor 1. Stained using Novocastra Thyroid Transcription Factor-1 for BON D (PA0364, Clone SPT24) with BON D Polymer Refine Detection™ (DS9800). Right. http://www.leicabiosystems.com/fileadmin/downloads_lbs/Leica... Zusammenfassung und Erläuterung Immunhistochemische Techniken eignen sich zum Nachweis des Vorhandenseins von Antigenen in Geweben und Zellen (siehe “Verwendung der Bond Reagenzien” in Ihrer Bond Benutzerdokumentation). Bond Polymer Refine Detection verwendet eine neuartige kontrollierte Polymerisationstechnologie zur Herstellung polymerer HRP-Linker-Antikörperkonjugate. Das Nachweissystem vermeidet die Verwendung von Streptavidin und Biotin und eliminiert daher unspezifische Färbung als Ergebnis des Vorhandenseins endogenen Biotins. https://www.leicabiosystems.com/fileadmin/img_uploads/novoca... |
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Reference Reference information: Purpose/Policy Statement: The demonstration of antigens in tissue and cells by immunostaining is a process involving first, the binding of an antibody to an antigen of interest and second visualization of the bound antibody by a biotin-free, polymeric horseradish peroxidase (HRP). The Bond Polymer Refine Detection system detects tissue- bound mouse and rabbit IgG and some mouse IgM primary antibodies. The detection kit is intended for staining sections of formalin-fixed, paraffin-embedded tissue on the Bond automated system. It utilizes a novel controlled polymerization technology to prepare polymeric HRP linker antibody and avoids the use of streptavidin and biotin. Therefore, eliminates non-specific staining as a result of endogenous biotin. The Bond DAB enhancer precipitates as a copper salt at the reaction site producing a darker brown staining deposit. Bond Polymer Refine Detection Catalog # DS9800 200-300 tests works as follows: - The specimen is incubated with hydrogen peroxide to quench endogenous peroxidase activity - A post primary antibody solution enhances penetration of the subsequent polymer reagent - A poly-HRP anti-mouse/rabbit IgG reagent localizes the primary antibody - The substrate chromogen, 3.3’- diaminobenzidine (DAB), visualizes the complex with a brown precipitate - Hematoxylin counterstaining allows the visualization of cell nuclei Bond Polymer Refine Red Detection a biotin-free, polymeric alkaline phosphatase (AP)-linker antibody conjugate system. Catalog # DS9390 100 tests works as follows: - A user-supplied specific primary antibody is applied - Post primary IgG linker reagent localizes mouse antibodies - Poly-AP IgG reagent localizes rabbit antibodies - The substrate chromogen, Fast Red, visualizes the complex with a red precipitate - Hematoxylin counterstaining allows the visualization of cell nuclei http://www.pacificpathologyinc.com/Procedures/AP-HISTO/Autom... -------------------------------------------------- Note added at 51 mins (2017-12-01 19:47:44 GMT) -------------------------------------------------- It utilizes a novel controlled polymerization technology to prepare polymeric HRP linker antibody related to these steps apparently: - A poly-HRP anti-mouse/rabbit IgG reagent localizes the primary antibody - Poly-AP IgG reagent localizes rabbit antibodies -------------------------------------------------- Note added at 1 hr (2017-12-01 19:59:20 GMT) -------------------------------------------------- Polymer Refine Detection System (met gecontroleerde polymerisatie verfijnd detectiesysteem) |
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