intact cell assay

12:55 Feb 24, 2021
English to Polish translations [PRO]
Medical - Medical (general)
English term or phrase: intact cell assay
Badania dotyczące wiązania leku z receptorami: [drug] has shown lower functional potency in intact cell assays than might have been expected based on its affinity.
Ka De
Local time: 22:54


Summary of answers provided
2badanie żywych i nieuszkodzonych komórek
Frank Szmulowicz, Ph. D.


  

Answers


1 hr   confidence: Answerer confidence 2/5Answerer confidence 2/5
badanie żywych i nieuszkodzonych komórek


Explanation:
Cell viability in living cells should be >95 % for various experimental investigations, including cell respirometry. Viable cells (vce) are characterized by an intact plasma membrane barrier function. The total cell count (Nce) is the sum of viable cells (Nvce) and dead cells (Ndce). In contrast, the plasma membrane can be permeabilized selectively by mild detergents (digitonin), to obtain the mt-preparation of permeabilized cells used for cell ergometry. Living cells are frequently labelled as intact cells in the sense of the total cell count, but intact may suggest dual meanings of viable or unaffected by a disease or mitochondrial injury.

https://www.bioblast.at/index.php/Living_cells#:~:text=Viabl...

Semi-intact cells are cells that have undergone plasma membrane permeabilization by the cholesterol-dependent pore-forming cytolysin, streptolysin O (SLO).
https://link.springer.com/protocol/10.1007/978-1-4939-7598-3...


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Badanie to pozwala na odróżnienie komórek żywych i nieuszkodzonych od martwych lub uszkodzonych, poprzez określenie liczby komórek żywych, zabarwionych na czerwono w komorze hemocytometru,
bądź spektrofotometrycznie przez pomiar absorbancji roztworu uzyskanego
po rozpuszczeniu barwnika w 1% roztworze acetonu zawierającego 30% etanolu, po uprzednim usunięciu podłoża hodowlanego [4]. Innym rozwiązaniem technicznym wskazującym na brak integralności błon komórkowych, jest pomiar aktywności enzymu cytoplazmatycznego dehydrogenazy mleczanowej, LDH w podłożu hodowlanym
https://www.ptfarm.pl/pub/File/FP/6_2009/01__porownanie uzyt...

Frank Szmulowicz, Ph. D.
United States
Local time: 17:54
Specializes in field
Native speaker of: Native in EnglishEnglish, Native in PolishPolish
PRO pts in category: 2209
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