Gene deletions can also be applied in many different plant and human pathogenic fungi in order to decrease their pathogenicity and/or virulence for different applications. (ScienceDirect)

It is notable that targeted gene deletions generated via CRISPR/Cas9 and two guide RNAs resulted in the formation of correct junctions at high efficiency. (Future Science)

In the genomics era there is great demand for fast, simple high-throughput methods for gene deletion to study the roles of the large numbers of genes that are being identified. (PubMed)