Glossary entry (derived from question below)
English term or phrase:
each with ramp of 1 column volume
English answer:
with a ramp of 1 column volume between every two steps
Added to glossary by
ysun
Oct 30, 2009 19:26
14 yrs ago
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English term
each with ramp of 1 column volume
English
Science
Chemistry; Chem Sci/Eng
Context:
“The gradient is 15 % EtOAc in heptanes over 2 column volumes followed by a step gradient to 30% EtOAc then 50% and then 70% EtOAc for 3 column volumes each with ramp of 1 column volume.”
My understanding of the text is that the step gradient refers to 3 stages: 1 column volume of 30% EtOAc, then 2 column volumes of 50% EtOAc, and then 3 column volumes of 70% EtOAc.
Could you please tell me if my understanding is correct or not? Thank you very much!
“The gradient is 15 % EtOAc in heptanes over 2 column volumes followed by a step gradient to 30% EtOAc then 50% and then 70% EtOAc for 3 column volumes each with ramp of 1 column volume.”
My understanding of the text is that the step gradient refers to 3 stages: 1 column volume of 30% EtOAc, then 2 column volumes of 50% EtOAc, and then 3 column volumes of 70% EtOAc.
Could you please tell me if my understanding is correct or not? Thank you very much!
Change log
Nov 6, 2009 01:45: ysun changed "Edited KOG entry" from "<a href="/profile/32249">ysun's</a> old entry - "each with ramp of 1 column volume"" to ""ramp of 1 column volume between every two steps""
Responses
3 days 3 hrs
Selected
ramp of 1 column volume between each step
1) 15% for 2 column volumes
2) ramp (linear increase) from 15% to 30% over 1 column volume
3) 30% for 3 column volumes
4) ramp from 30% to 50% over 1 column volume
5) 50% for 3 column volumes
... etc.
2) ramp (linear increase) from 15% to 30% over 1 column volume
3) 30% for 3 column volumes
4) ramp from 30% to 50% over 1 column volume
5) 50% for 3 column volumes
... etc.
Note from asker:
| David, I think your explanation makes sense although the original text has some problems. Thank you very much for your help! |
4 KudoZ points awarded for this answer.
Comment: "David,
Thank you very much!"
Discussion