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  3. Biology (-tech,-chem,micro-)

scan (probe arrays)

Spanish translation: explorar

22:48 May 11, 2016
English to Spanish translations [PRO]
Medical - Biology (-tech,-chem,micro-)
English term or phrase: scan (probe arrays)
En un artículo titulado "Selective Inhibition of Inflammatory Gene Expression in Activated T Lymphocytes: A Mechanism of Immune Suppression by Thiopurines":

"Sample Preparation and **Scanning** for Affymetrix Gene Chip Expression Profiling. Total RNA was isolated from Ju.1 cells and purified using commercial affinity resin column kits [marca]. cDNA was prepared from a total of 10 μg of RNA pooled from three independent experiments to minimize the effect of random variation on the expression profiles. The purified cDNA was used as a template for in vitro transcription reaction for the synthesis of biotinylated cRNA using RNA transcript labeling reagent [marca]. Labeled cRNAs were then fragmented and hybridized onto the U133A array. Appropriate amounts of fragmented cRNA and control oligonucleotide B2 were added along with control cRNA (BioB, BioC, and BioD), herring sperm DNA, and bovine serum albumin to the hybridization buffer. The hybridization mixture was heated at 99°C for 5 min followed by incubation at 45°C for 5 min before injecting the sample into the microarray. Then, the hybridization was carried out at 45°C for 16 h with mixing on a rotisserie at 60 rpm. After hybridization, the solutions were removed and the arrays were washed and then stained with streptavidin-phycoerythrin [marca]. After washes, probe arrays were **scanned** using the Genechip system confocal scanner [marca]. The quality of the fragmented biotin labeled cRNA in each experiment was evaluated before hybridizing onto the U133A expression array by both gel electrophoresis and hybridizing a fraction of the sample onto test-3 array as a measure of quality control."

Había usado analizar, pero luego dice:

"Gene Chip Data analysis. GeneChip 5.0 (Affymetrix) was used to **scan** and quantitatively analyze the scanned image. Once the probe array has been scanned, GeneChip software automatically calculates intensity values for each probe cell and makes a presence or absence call for each mRNA."

Pensé también en leer (dado que scanner en este contexto parece traducirse como lector óptico). ¿Cuál es la traducción más adecuada de "scan" dentro de este contexto? ¿explorar?

Muchas gracias por adelantado
Yvonne Becker
Local time: 03:05
Spanish translation:explorar
Explanation:
... donde es explorado ...
Selected response from:

Maximo Wilhelm Muñoz
Guatemala
Local time: 01:05
Grading comment
Muchas gracias
4 KudoZ points were awarded for this answer



Summary of answers provided
5 +1explorar
Maximo Wilhelm Muñoz
4..fueron analizados utilizando el sistema confocal de barrido..
VICTORDIAZ
3detección/análisis/revisión(micro-arreglos DNA en chip)
lugoben


  

Answers


5 mins   confidence: Answerer confidence 5/5 peer agreement (net): +1
explorar


Explanation:
... donde es explorado ...

Maximo Wilhelm Muñoz
Guatemala
Local time: 01:05
Native speaker of: Native in SpanishSpanish, Native in EnglishEnglish
PRO pts in category: 4
Grading comment
Muchas gracias

Peer comments on this answer (and responses from the answerer)
agree  Irenefranck
5 mins
  -> Thank you for your agree Irene. :)
Login to enter a peer comment (or grade)

16 hrs   confidence: Answerer confidence 3/5Answerer confidence 3/5
detección/análisis/revisión(micro-arreglos DNA en chip)


Explanation:
The robotic instruments for making microarrays are collectively known as “arrayers”. The term “spotter” is often used interchangeably, although it is technically limited to instruments that deposit (or “spot”) cDNA probes onto a microscope slide or other substrate. These probes are synthesized off-chip and are temporarily stored in a microwell plate or other receptacle from which they are sampled. Most commercially available arrayers fall into this category.
http://pubs.acs.org/subscribe/journals/mdd/v04/i05/html/05zu...

Tecnología de microarrays (chips de ADN o ARN)
La tecnología de microarrays es una tecnología en desarrollo para estudiar la expresión de muchos genes a la vez. Consiste en colocar miles ...
https://www.genome.gov/glossarys/index.cfm?id=125

The scanner is a sophisticated confocal laser fluorescence scanner which measures fluorescence intensity emitted by the labeled cRNA bound to the probe arrays. The scanner uses an argon-ion laser to excite the fluorophores at 488nm. The scanner can focus the laser to a spot size of less than 4 microns and can detect as few as 400 phycoerythrin molecules in a 20 x 20 .m probe site.
http://icahn.mssm.edu/research/labs/life-science-technology-...

Los experimentos de micro-arreglos DNA constan de una primera etapa
https://www.researchgate.net/profile/Ricardo_Bringas/publica...

lugoben
Local time: 03:05
Native speaker of: Spanish
PRO pts in category: 60
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18 hrs   confidence: Answerer confidence 4/5Answerer confidence 4/5
..fueron analizados utilizando el sistema confocal de barrido..


Explanation:
En este caso la primera vez que usa "scanned" sugiero que la traduzcas como analizadas y ya en la descripción del aparato utilizar "barrido".
Realmente se analiza la muestra por barrido, pero no creo que se pueda utilizar "barriendo" la muestra, cuando realmente se está analizando por barrido.

Ejemplo:
El CLSM (Microscopio Confocal Láser de Barrido) combina el microscopio de fluorescencia con imagen electrónica y puntos de luz suministrados que nos permite obtener imágenes en tres-dimensiones sobre el objeto deseado.

Por ejemplo muestras de alimentos de determinado grosor pueden ser analizados por el CLSM para obtener información estructural


    https://es.wikipedia.org/wiki/Microscopio_confocal_l%C3%A1ser_de_barrido
VICTORDIAZ
Mexico
Local time: 01:05
Works in field
Native speaker of: Native in SpanishSpanish
PRO pts in category: 4
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