Jul 20, 2007 18:10
16 yrs ago
English term
re-probed
English to Chinese
Medical
Medical (general)
Could you please tell me what "re-probed" means here? Thank you in advance!
Text:
Protein concentration was determined by preparing lysates and Western blotting using antibodies to Core 1. The blots were re-stripped and re-probed using anti-tubulin antibodies as a loading control.
Text:
Protein concentration was determined by preparing lysates and Western blotting using antibodies to Core 1. The blots were re-stripped and re-probed using anti-tubulin antibodies as a loading control.
Proposed translations
(Chinese)
| 4 +2 | 再检测 |
Lu Zou
|
| 4 +1 | 再杂交 |
Shaunna (X)
|
| 4 +1 | 再次探针检测 |
Jianming Sun
|
Proposed translations
+2
4 hrs
Selected
再检测
洗脱后再以微管蛋白抗体检测，做参照之用。
4 KudoZ points awarded for this answer.
Comment: "The answers, suggestions and links provided by all of you are really very helpful. Thank you all!"
+1
11 mins
Shaunna (X)
United States
Native in English
&
Chinese
Specializes in field
Related KudoZ points
:
42
再杂交
就是杂交后的膜，经过洗脱出去结合的抗体之后，再用一种抗体进行杂交检测。
--------------------------------------------------
Note added at 19 mins (2007-07-20 18:29:24 GMT)
--------------------------------------------------
就重复性而言：尼龙膜可反复用于分子杂交，杂交后，探针分子可经碱变性被洗脱下来；硝酸纤维素膜不能重复使用；PVDF膜可以重复使用。
http://www.labbase.net/ShowNewsDetails_3345.html
--------------------------------------------------
Note added at 19 mins (2007-07-20 18:29:24 GMT)
--------------------------------------------------
就重复性而言：尼龙膜可反复用于分子杂交，杂交后，探针分子可经碱变性被洗脱下来；硝酸纤维素膜不能重复使用；PVDF膜可以重复使用。
http://www.labbase.net/ShowNewsDetails_3345.html
Peer comment(s):
| agree |
Jing Nie
: http://bio.hengqian.com/html/2007/2-10/r162317451.shtml , www.kangchen.com.cn/PDF/KC-420.pdf
5 hrs
|
|
Thank you Niejing! //刚从我的email alert里看到你本来是先给我提出来核酸用杂交才合适，蛋白用不合适，其实那是很对的，我看到Lu的答案就反应过来了，是我考虑不周。难得你又去考证，谢谢。
|
+1
5 hrs
Jianming Sun
Native in Chinese
Specializes in field
Accurate -- Reliable -- Fast
Native in: Chinese 
Works in: English to Chinese, Chinese to English, German to Chinese, and 3 more.
Related KudoZ points
:
172
再次探针检测
剥离，再次探针检测，以及免疫测定方法如Western blot 和 dot-blots的再次分析
http://www.supermed.com.cn/htm/new/43.htm
--------------------------------------------------
Note added at 6 hrs (2007-07-21 00:11:37 GMT)
--------------------------------------------------
以上的说法在在原理上讲是成立的，但认为可能还要将探针的意思表达出来。
--------------------------------------------------
Note added at 6 hrs (2007-07-21 00:12:06 GMT)
--------------------------------------------------
以上的说法在在原理上讲是成立的，但我认为可能还要将探针的意思表达出来。
--------------------------------------------------
Note added at 18 hrs (2007-07-21 12:34:08 GMT)
--------------------------------------------------
Re-ProbeTM 优点
· 用多次探针反复Western blots 测定
· 再次分析和改正不满意的Western blots
· 保留困难得到的实验样本和试剂
· 节省了重新电泳和转移过程的时间
http://www.genecool.com/bbs/18722/ShowPost.aspx
--------------------------------------------------
Note added at 18 hrs (2007-07-21 12:58:46 GMT)
--------------------------------------------------
Western blotting 是在从混合蛋白质中检测微量特定蛋白质的一种方法。简单、通俗地说就是先用凝胶电泳方法分离蛋白质，并保留电泳区带的原状，然后将其转移到滤膜上，再以带有放射性同位素的特定抗体（即所谓探针）进行检测，也就是利用探针与特定蛋白质结合的特性检测该蛋白质。探针与特定蛋白质结合后，因其上有放射性同位素可通过照相的方法使其显示；如不能结合将被洗掉。所谓RE-PROP，也就是将膜上蛋白质-探针结合体的探针剥离掉后，再用探针检测一次，以再次分析和改正不满意的试验，或作为对照。
http://www.supermed.com.cn/htm/new/43.htm
--------------------------------------------------
Note added at 6 hrs (2007-07-21 00:11:37 GMT)
--------------------------------------------------
以上的说法在在原理上讲是成立的，但认为可能还要将探针的意思表达出来。
--------------------------------------------------
Note added at 6 hrs (2007-07-21 00:12:06 GMT)
--------------------------------------------------
以上的说法在在原理上讲是成立的，但我认为可能还要将探针的意思表达出来。
--------------------------------------------------
Note added at 18 hrs (2007-07-21 12:34:08 GMT)
--------------------------------------------------
Re-ProbeTM 优点
· 用多次探针反复Western blots 测定
· 再次分析和改正不满意的Western blots
· 保留困难得到的实验样本和试剂
· 节省了重新电泳和转移过程的时间
http://www.genecool.com/bbs/18722/ShowPost.aspx
--------------------------------------------------
Note added at 18 hrs (2007-07-21 12:58:46 GMT)
--------------------------------------------------
Western blotting 是在从混合蛋白质中检测微量特定蛋白质的一种方法。简单、通俗地说就是先用凝胶电泳方法分离蛋白质，并保留电泳区带的原状，然后将其转移到滤膜上，再以带有放射性同位素的特定抗体（即所谓探针）进行检测，也就是利用探针与特定蛋白质结合的特性检测该蛋白质。探针与特定蛋白质结合后，因其上有放射性同位素可通过照相的方法使其显示；如不能结合将被洗掉。所谓RE-PROP，也就是将膜上蛋白质-探针结合体的探针剥离掉后，再用探针检测一次，以再次分析和改正不满意的试验，或作为对照。
Something went wrong...