English to French Science Genetics DNA endonuclease

Step 1 - Prepare PCR amplicons from mutant (test) and normal (reference) DNA.
Step 2 – Continuing on from the final PCR amplification cycle the reaction is heated to melt all double strands and then cooled slowly for optimal formation of hetero- and homoduplexes.
Step 3 - Treat the annealed heteroduplex/homoduplex mixture with XXX. The reference DNA alone, treated similarly, serves as a background control.
Step 4 - Analyze the DNA fragments with the XXX System. The formation of new cleavage products, due to the presence of one or more mismatches, is indicated by the

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