English to Russian Medical Biology (-tech,-chem,micro-) лабораторная диагностика

Product Code CK113
Description of test
The IBD ELISA kit will measure the amount of antibody to IBD in the serum of chickens. Microtitre plates have been pre-coated with inactivated IBD antigen. Chicken serum samples are diluted and added to the microtitre wells where any anti-IBD antibodies present will bind and form an antigen-antibody complex. Non specific antibodies and other serum proteins are then washed away. Anti-chicken IgG labelled with the enzyme alkaline phosphatase is then added to the wells and binds to any chicken anti-IBD antibodies bound to the antigen. After another wash to remove unreacted conjugate, substrate is added in the form of pNPP chromogen. A yellow colour is developed if anti-IBD antibody is present and the intensity is directly related to the amount of anti-IBD present in the sample.

Reagents provided
1. IBD Coated plates. Inactivated viral antigen on microtitre plates.
2. Conjugate reagent Anti-Chicken: Alkaline Phosphatase in Tris bufler with protein stabilisers, inert red dye and sodium azide preservative (0.1 % w/v).
3. Substrate tablets. PNPP (p-Nitrophenyl Phosphate) tablets to dissolve with Substrate buffer.
4. Substrate buffer reagent. Diethanolamine bufler with enzyme co-factors.
5. Stop solution. Sodium Hydroxide in Diethanolamine bufier.
6. Sample diluent reagent. Phosphate bufler with protein stabilisers and sodium azide preservative (0.2 % w/v).
7. Wash buffer. Powdered Phosphate Buffered Saline with Tween.
8. Negative control. Specific Pathogen Free serum in Phosphate buffer with protein stabilisers and sodium azide preservative (0.2 % w/v).
9. Positive control. Antibodies specific to IBD in Phosphate buffer with protein stabilisers and sodium azide preservative (0.2 % w/v).

Materials and equipment required (not provided with kit)
Precision pipettes and disposable tips
8 or 12 channel pipette/repeater pipette
Plastic tubes or dilution plate for sample dilution
Distilled or deionized water
Microtiter plate reader with 405 nm filter
Microtiter plate washer