Feb 11, 2018 22:28
6 yrs ago
English term
Development Solution
English to Romanian
Medical
Medical: Instruments
Endocrinologie
Graves’ disease is a common autoimmune disorder with the most common manifestation being hyperthyroidism caused by antibodies binding to and activating the TSH-R on the follicular cells of the thyroid. Therefore, the vast majority of patients newly diagnosed with Graves’ disease have detectable anti-TSH-R antibodies in the serum.
EliA anti-TSH-R is a competitive enzyme immunoassay. The EliA anti-TSH-R Wells are coated with human recombinant TSH-R and can be used for measuring of patients' samples as well as calibrators and curve controls.
Anti-TSH-R antibodies in patients' samples, calibrators and controls bind to the coated human TSH-R. After washing away non-bound components, enzyme-labeled recombinant antibodies (EliA anti-TSH-R Conjugate) are added to form a TSH-R-conjugate complex, if the TSH-R is not blocked by antibodies from patients' serum, calibrator or control.
After incubation, non-bound conjugate is washed away and the bound complex is incubated with Development Solution. After stopping the conjugate-enzyme reaction, the fluorescence activity, corresponding to the bound enzyme-labelled conjugate, is measured. The lower the response value of the fluorescence, the less conjugate is bound to coated TSH receptor, because more antibodies from patients' samples, calibrator or control are bound to coated TSH receptor.
EliA anti-TSH-R is a competitive enzyme immunoassay. The EliA anti-TSH-R Wells are coated with human recombinant TSH-R and can be used for measuring of patients' samples as well as calibrators and curve controls.
Anti-TSH-R antibodies in patients' samples, calibrators and controls bind to the coated human TSH-R. After washing away non-bound components, enzyme-labeled recombinant antibodies (EliA anti-TSH-R Conjugate) are added to form a TSH-R-conjugate complex, if the TSH-R is not blocked by antibodies from patients' serum, calibrator or control.
After incubation, non-bound conjugate is washed away and the bound complex is incubated with Development Solution. After stopping the conjugate-enzyme reaction, the fluorescence activity, corresponding to the bound enzyme-labelled conjugate, is measured. The lower the response value of the fluorescence, the less conjugate is bound to coated TSH receptor, because more antibodies from patients' samples, calibrator or control are bound to coated TSH receptor.
Proposed translations
(Romanian)
5 | soluţie agent de developare/ soluție de conjugat) | Manuela C. |
Proposed translations
10 hrs
Selected
soluţie agent de developare/ soluție de conjugat)
- pipete automate de volume diferite(1 -500ul) şi vârfuricorespunzătoare;- pipetă multichannel şi vârfuri corespunzătoare;- placi de microtitrare corespunzătoare modelului experimental;- pisetă de plastic;- spectrofotometru;- cuve plastic pentru pipeta multichannel; REACTIVI:- soluţie agent de developare (conjugat enzimă-anticorp/antigen);- soluţie antigen;- soluţie anticorpi de testat;- TFS (tampon fosfat salin);- apă distilată sau deionizata;
https://fr.scribd.com/doc/39660752/Tehnica-ELISA-complet
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Note added at 10 hrs (2018-02-12 09:23:52 GMT)
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Definiție aici:http://www.inter-2t.phadia.com/PageFiles/35254/IDM_Reference...
https://fr.scribd.com/doc/39660752/Tehnica-ELISA-complet
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Note added at 10 hrs (2018-02-12 09:23:52 GMT)
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Definiție aici:http://www.inter-2t.phadia.com/PageFiles/35254/IDM_Reference...
4 KudoZ points awarded for this answer.
Comment: "Multumesc"
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